Surface Plasmon Resonance
SPR is a phenomenon in which the reflected light disappears at a specific angle of incidence when light is incident on the side of the prism on which the gold thin film is placed. When biomolecules bind on the sensor chip, the surface refractive index changes and the angle of the reflected light shifts. Molecular interaction is monitored by acquiring sensorgrams that record this angle change in real time.
SPR measures the binding of various molecules such as antigen-antibody, protein, nucleic acid, carbohydrate, fat, cell, and virus.
In addition, label-free real-time analysis enables kinetic analysis of intermolecular association affinity (affinity) as well as
association rate constant and dissociation rate constant.