The most used
basic analysis running buffer

10X HBST buffer (50ml, 500ml)

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10-fold concentrated HBST buffer

Running buffer for binding analysis

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This buffer is filtered.

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ingredient

150mM NaCl

10mM HEPES

0.002% T20 surfactants

pH7.4

Amine coupling kit

A set of reagents for approximately 100 uses for ligand immobilization.

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Activation reagent 1: Activation buffer (NHS, 50ml)

Activation reagent 2: EDC, 1 g

Blocking reagent: Quenching buffer, 50ml

Borate elution buffer, 50ml

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Applied sensor chip

COOH-Au chip

CMDxxxM

HCxxxM

Ligand​ immobilization buffer

Acetate buffer for each pH for ligand immobilization. When used with an amine coupling kit, ligands can be effectively immobilized on a COOH functionalized chip.

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5mM acetate buffer pH4.0, 50ml

5mM acetate buffer pH4.5, 50ml

5mM acetate buffer pH5.0, 50ml

5mM acetate buffer pH5.5, 50ml

Coupling kit for NTA functionalized sensor chip

A set of reagents for approximately 100 uses for the immobilization of Histag ligands.

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Running buffer, 50ml

Activation reagent: Nikel ion solution, 50ml

EDTA regeneration buffer, 50ml

Imidazole regeneration buffer, 50ml

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Applied sensor chip

NTA-Au chip

NiDxxxM

NiHCxxxM

Regeneration buffers

Main regeneration reagents regenerate the ligand-immobilized sensor chip by desorbing the analyte bound to the ligand.

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10mM Glycine-HCl pH1.5, 50ml

10mM Glycine-HCl pH2.0, 50ml

10mM Glycine-HCl pH2.5, 50ml

10mM Glycine-HCl pH3.0, 50ml

50 mM NaOH, 50 ml

0.5% SDS, 50ml