The most used
basic analysis running buffer
10X HBST buffer (50ml, 500ml)
10-fold concentrated HBST buffer
Running buffer for binding analysis
This buffer is filtered.
ingredient
150mM NaCl
10mM HEPES
0.002% T20 surfactants
pH7.4
Amine coupling reagent for
COOH functionalized sensor chip
Amine coupling kit
A set of reagents for approximately 100 uses for ligand immobilization.
Activation reagent 1: Activation buffer (NHS, 50ml)
Activation reagent 2: EDC, 1 g
Blocking reagent: Quenching buffer, 50ml
Borate elution buffer, 50ml
Applied sensor chip
COOH-Au chip
CMDxxxM
HCxxxM
Ligand immobilization buffer
Acetate buffer for each pH for ligand immobilization. When used with an amine coupling kit, ligands can be effectively immobilized on a COOH functionalized chip.
5mM acetate buffer pH4.0, 50ml
5mM acetate buffer pH4.5, 50ml
5mM acetate buffer pH5.0, 50ml
5mM acetate buffer pH5.5, 50ml
Reagents related to NTA-Histag binding
Coupling kit for NTA functionalized sensor chip
A set of reagents for approximately 100 uses for the immobilization of Histag ligands.
Running buffer, 50ml
Activation reagent: Nikel ion solution, 50ml
EDTA regeneration buffer, 50ml
Imidazole regeneration buffer, 50ml
Applied sensor chip
NTA-Au chip
NiDxxxM
NiHCxxxM
Regeneration buffers
Main regeneration reagents regenerate the ligand-immobilized sensor chip by desorbing the analyte bound to the ligand.
10mM Glycine-HCl pH1.5, 50ml
10mM Glycine-HCl pH2.0, 50ml
10mM Glycine-HCl pH2.5, 50ml
10mM Glycine-HCl pH3.0, 50ml
50 mM NaOH, 50 ml
0.5% SDS, 50ml
Maintanance reagents
Desorb reagent for maintenance to decontaminate device tubing.
Desorb solution1
0.5% SDS, 500ml
Desorb solution2
50mM Glycine pH9.5, 500ml