The most used
basic analysis running buffer

running_buffers.jpg

10X HBST buffer (50ml, 500ml)

10-fold concentrated HBST buffer

Running buffer for binding analysis

This buffer is filtered.

ingredient

150mM NaCl

10mM HEPES

0.002% T20 surfactants

pH7.4

Amine coupling reagent for
COOH functionalized sensor chip

amine coupling.png

Amine coupling kit

immobilization_reagents_and_kits.jpg

A set of reagents for approximately 100 uses for ligand immobilization.

Activation reagent 1: Activation buffer (NHS, 50ml)

Activation reagent 2: EDC, 1 g

Blocking reagent: Quenching buffer, 50ml

Borate elution buffer, 50ml

Applied sensor chip

COOH-Au chip

CMDxxxM

HCxxxM

Ligand​ immobilization buffer

immobilization_reagents_and_kits.jpg

Acetate buffer for each pH for ligand immobilization. When used with an amine coupling kit, ligands can be effectively immobilized on a COOH functionalized chip.

5mM acetate buffer pH4.0, 50ml

5mM acetate buffer pH4.5, 50ml

5mM acetate buffer pH5.0, 50ml

5mM acetate buffer pH5.5, 50ml

Reagents related to NTA-Histag binding

NTA-his.png

Coupling kit for NTA functionalized sensor chip

capture_reagents_and_kits.jpg

A set of reagents for approximately 100 uses for the immobilization of Histag ligands.

Running buffer, 50ml

Activation reagent: Nikel ion solution, 50ml

EDTA regeneration buffer, 50ml

Imidazole regeneration buffer, 50ml

Applied sensor chip

NTA-Au chip

NiDxxxM

NiHCxxxM

Regeneration buffers

regeneration_buffers_and_kits.jpg

Main regeneration reagents regenerate the ligand-immobilized sensor chip by desorbing the analyte bound to the ligand.

10mM Glycine-HCl pH1.5, 50ml

10mM Glycine-HCl pH2.0, 50ml

10mM Glycine-HCl pH2.5, 50ml

10mM Glycine-HCl pH3.0, 50ml

50 mM NaOH, 50 ml

0.5% SDS, 50ml

Maintanance reagents

reagents_for_instrument_maintenance.jpg

Desorb reagent for maintenance to decontaminate device tubing.

Desorb solution1

0.5% SDS, 500ml

Desorb solution2

50mM Glycine pH9.5, 500ml