SPR sensor chip

Sensor chips

Sensor Chip Selection Guide

SPR sensor chips offer a wide variety of surfaces depending on the analyte, experimental method, etc.

Are you having trouble finding the right sensor chip for your research purposes?

Please refer to the sensor chip selection guide below.

SPR sensor chip selection guide

2D (Planar) Au sensor chip

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Because the iMSPR system has sufficient sensitivity, in the case of molecules with relatively large analytes (more than about 5 kDa) - proteins, cells, bacteria, viruses, exosomes, etc. - a monolayer of linker material that can immobilize ligands is formed. Satisfactory results can also be obtained with the 2D Au sensor chip. In particular, in the case of antibody drugs, it is a very suitable application field because of its large signal and high affinity.

3D (membrane) Au sensor chip

Although iMSPR has sufficient sensitivity, it is difficult to obtain a sufficient signal in the case of small molecule compounds and peptides (about 5kDa or less) in a monolayer sensor chip. Therefore, it is recommended to analyze using a membrane-type surface sensor chip (3D Au sensor chip) that can more sufficiently immobilize the ligand.

iClubio provides HC Au sensor chips based on linear synthetic polycarboxylates with better performance as well as CMD Au sensor chips of the carboxymethylated dextran series, which are the most used in the past.

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3D Carboylate Dextran (CMD) Sensor Chip

The dextran sensor chip was first commercialized in the early 1990s by applying the well-optimized solid-phase surface technology in affinity chromatography. Therefore, so far, the largest amount of data has been accumulated using SPR. Therefore, there are many references and results, so it is very advantageous in terms of reproducing similar experiments.

The representative dextran sensor chip provided by iCLUBIO is CMD200M. The dextran membrane layer is about 200 nm thick and the chip has a medium surface density.

Linear polymer, HC Sensor chip

SPR sensor chip linear polymer surface
SPR sensor chip linear polymer surface

The HC sensor chip is a linear polymer polycarboxylate hydrogel surface.

 

The HC sensor chip is characterized by very high hydrophilicity and has a very high immobilization capacity compared to the dextran surface.

 

In addition, due to its relatively simple and uniform chemical and structural features, it exhibits very little non-specific binding and high sensitivity.

Recommended sensor chip for
each type of ligand-analyte

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