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SPR sensor chip

sensor chips

Sensor Chip Selection Guide

icluebio is designed to be applied to a variety of applications.

We offer a wide range of sensor chip surfaces - types, functional groups, densities and thicknesses.

The surface of the sensor chip have to be determined the type of analyte, the size of the analyte, the ligand immobilization method, non-specific adsorption, etc.

Recommended sensor chip for each type of ligand-analyte

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2D (Planar) Au sensor chip

Since the iMSPR system has sufficient sensitivity, in the case of molecules with relatively large analytes (more than about 5 kDa) - proteins, cells, bacteria, viruses, exosomes, etc. - a monolayer of linker material that can immobilize ligands is formed Satisfying results can also be obtained with a sensor chip (Planar Au sensor chip). In particular, in the case of antibody drugs, it is a very suitable application field because of its large signal and high affinity.

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3D (membrane) Au sensor chip

Although iMSPR has sufficient sensitivity, it is difficult to obtain a sufficient signal in the case of small molecule compounds and peptides (about 5kDa or less) in a monolayer sensor chip. Therefore, it is recommended to analyze using a membrane-type surface sensor chip (3D Au sensor chip) that can more sufficiently immobilize the ligand.

icluebio provides HC Au sensor chips based on linear synthetic polycarboxylates with better performance as well as CMD Au sensor chips of the carboxymethylated dextran series, which are the most used in the past.

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3D Carboylate Dextran (C-Dex) Sensor Chip

The dextran sensor chip was first commercialized in the early 1990s by applying a solid-phase surface technology that was already well-optimized in affinity chromatography. Therefore, so far, the largest amount of data has been accumulated using SPR. Therefore, there are many references and results, so it is very advantageous in terms of reproducing similar experiments.

icluebio's dextran sensor chip is available in various thicknesses. It can be selected according to the molecular weight of the analyte and non-specific adsorption properties.

Linear polymer, HC Sensor chip

SPR sensor chip linear polymer surface
SPR sensor chip linear polymer surface

There is a lot of data accumulated over a long period of time of the dextran chip, and it plays a faithful role as a 3D sensor chip, but since dextran is a naturally-derived material, it is difficult to manufacture the same repeatedly because of its irregular structure. Because they are relatively large molecules in the hydrated state, they not only interfere with the free diffusion of analytes, but they also occupy a significant volume of the SPR evanescent field, reducing the signal-to-noise ratio.

The newly developed linear polymer sensor chip is a sensor chip that retains the performance of the membrane sensor chip and reinforces the shortcomings of the dextran sensor chip. It can be fabricated more reproducibly, shows a higher signal-to-noise ratio, has improved diffusion properties, and further reduces non-specific adsorption. The representative sensor chip is HC1000. It is a sensor chip that is very specialized for screening, concentration measurement, and kinetics evaluation of small molecule analytes.

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