As can be seen in the table above, the association rate constant (ka), dissociation rate constant (kd), and binding affinity (KD) all do not show significant differences depending on the analysis method. So, in which cases are each of the two analysis methods more suitable?

As mentioned above, when it is difficult to optimize the regeneration conditions, it is recommended to use the SCK analysis method. In order to find the appropriate regeneration conditions, you need to test various solutions with properties such as acid, base, ion, and hydrophobicity. In addition, in order to prevent damage to the ligand, you need to start with a solution with weak properties and gradually increase the strength while testing. For example, when using an acidic solution as a regeneration solution, you need to start with a high pH and gradually lower it to find the conditions for regeneration without damaging the ligand. Therefore, optimizing the regeneration conditions may require many tests, and even after this process, you may not be able to find the appropriate regeneration conditions. In this case, we recommend using the SCK analysis method that does not require a regeneration process. In addition, in SCK analysis, the dissociation of analytes of multiple concentrations in front is observed briefly, and the dissociation of the analyte of the highest concentration at the end is observed for a long time to evaluate the dissociation tendency. This helps reduce the total kinetic analysis time.
However, depending on the characteristics of the interaction, it may be difficult to evaluate the binding with only SCK, which has less information than MCK. In particular, the MCK method is suitable when the binding is complex. If one of the curves of multiple concentration analytes is bad, MCK has the advantage of being able to evaluate by removing the bad curve from the curve overlay.

icluebio provides S/W that can analyze MCK and SCK.
Selecting an evaluation method appropriate for the interaction to be analyzed can not only provide efficient analysis, but also further expand the understanding of the kinetic characteristics of the interaction.